Infrequent K-ras codon 12 mutation in serrated adenomas of human colorectum

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Infrequent K-ras codon 12 mutation in serrated adenomas of human colorectum.

BACKGROUND Serrated adenoma is a new morphological subtype of colorectal adenoma. The lesion provides a distinct morphological route to carcinoma, but the underlying genetic changes have not yet been investigated. AIMS To determine the frequency of K-ras mutation in serrated adenoma. METHODS The frequency of K-ras codon 12 point mutation in 20 serrated adenomas, five atypical hyperplastic p...

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codon 12 k-ras mutation detection in iranian patients with colorectal cancer using pcr-rflp method

colorectal cancer (crc) is one of the most common cancers worldwide. understanding of the tumor behavior, in a much closer look, at the molecular level, results in a more effective treatment and accurate prognosis of the disease. from among various genes altered in colorectal cancer k-ras is assumed to be of diagnostic and prognostic significance. k-ras mutations are believed to be a critical e...

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Significance of the K-ras Gene Codon 12 Point Mutation in Stomach Cancer in Southern India

The frequency and clinicopathologic significance of the K-ras gene point mutation in stomach cancer remain to be defined. We investigated the frequency of K-ras codon 12 point mutations in stomach cancer using a polymerase chain reaction (PCR)-based method in 94 samples and 100 age and sex matched controls. The overall frequency of K-ras codon 12 point mutations in stomach cancer was 3.19% (3/9...

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p53 expression and K-ras mutation in colorectal adenomas.

The frequency of p53 overexpression and K-ras codon 12 mutation was investigated in a series of colorectal adenomas. p53 was detected by immunohistochemistry in only 5% of tumours, whereas K-ras mutation was found in eight of 30 adenomas examined. In vitro, mutant p53 and ras genes cooperate to transform primary rat cells into a tumourigenic cell line. The presence of both p53 overexpression an...

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K-ras oncogene codon 12 point mutations in testicular cancer.

A significant association between N-ras oncogene activating point mutations and testicular cancer has recently been reported. We have studied DNA samples from the blood and fresh tumor tissues of 17 Norwegian testicular cancer patients (11 seminomas/6 nonseminomas). Point mutations in K-ras-2 and N-ras exons 1 and 2 were studied by denaturing gradient gel electrophoresis (DGGE) and by oligonucl...

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ژورنال

عنوان ژورنال: Gut

سال: 1998

ISSN: 0017-5749,1468-3288

DOI: 10.1136/gut.42.5.680